CHO cells have been the industry workhorse for producing therapeutic proteins for the last several decades. There has been a lot of focus on cell growth and antibody productivity to obtain high titers to meet clinical/commercial demands. Improvements in media and feed formulations have been one of the major contributors for obtaining high titer fed batch processes. We evaluated different feeding schemes on cell growth and cell productivity in the ambr15 system. The ambr15 is a high throughput, automated bioreactor system for 24 parallel cultivations at the 10 –15 mL microbioreactor scale. Lowering the feed led to increased cell growth and spent media analysis led to the identification of limiting media components. Supplementation of the limiting components, while lowering the feed, improved the productivity. Furthermore, we were able to successfully implement this process at bench scale.

Modified feed formulation strategy for increased mAb productivity

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.