Variability in trace metal levels within CHO cell culture processes is known to impact CHO cell metabolism, with potential effects on product quality and process performance.  Sources of this variability may include raw materials used in the bioreactor media and potential leaching from process equipment. The ranges in trace metal levels were quantified across multiple lots of a chemically defined media, and potential introduction of trace metals from equipment contact was quantified under worst-case process conditions for metals leaching. The observed ranges for each metal were compared to theoretical process values (i.e., media formulation targets), and experimentally evaluated for their impact on multiple CHO cell culture processes.  Control strategies were developed to limit trace metal variability to a range demonstrated to be acceptable to the CHO cell culture processes.<br/>

Impact of trace metal variability on CHO cell culture processes and application of mitigating control strategies

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.