The Berkeley Light’s Beacon is a nanofluidic cell culturing device that allows simultaneous culturing of thousands clonally isolated cells on a single chip. We have previously reported the utility of this technology to Cell Line Development through its ability to generate biologics manufacturing quality CHO cell lines with improved assurances and documentation of clonal derivation.  Here, we report that the Beacon can be employed to isolate cell lines with higher specific productivity, shorter cell line development timelines, and improved prediction of population dynamics.<br/>The differentiating application of the technology is its ability to perform light and fluorescent-based live cell imaging assays at multiple time points to garner phenotypical information about each clonally isolated population prior to export and expansion for additional screening or genetic characterization.  Berkeley Light’s commercially available developed Spotlight® assay utilizes a fluorescently conjugated anti-Fc probe to detect secreted protein in individual pens.  The assay allows quantification of the steady-state secreted protein level in each pen at a specific time point. It is not clear whether measured secreted protein produced by a few cells in a nanofluidic environment at an early timepoint is predictive of future productivity at large-scale productions with many orders of magnitude more cells, in a different production environment, and over several days.  Using a retrospective multi-regression approach, we find Spotlight® scores are highly predictive of cell specific productivity. With the ability to predict specific productivity at an early stage with a few cells, we also demonstrate how single cell subcloning processes can be initiated earlier in the typical cell line development campaign. This has enabled the generation of highly productive subclones in under two months. Combined, a cell line development platform integrated with the Beacon technology platform enables production of stable clonally-derived cell banks with higher productivity, shorter development timelines, and improved phenotypic characterization.<br/>

Accelerating cell line development with rapid single cell and population level discovery on the Berkeley Lights Beacon

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.