The usage of low-cost readily-available or even disposable single-use filter or membranes in biopharma applications are becoming an attractive alternative for large-scale manufacturing, eliminating the need for the development and validation of intermediate cleaning cycles. In this frame, fiber-based membranes are also becoming of particular significance, where, however, beside of high-enough adsorption-specific sites and tailored pore size distribution, efficient regeneration together with no/low swelling, which directly impacts on the filtration performance in terms of permeability and retention, is still the main challenge.<br/>The aim of this work was to ascertain the morphological (SEM, Mercury), surface (charge/potentiometric titration, hydrophobicity/ZP), swelling/shrinkage and mechanical (strength, pressure stability) properties of membranes prepared from carboxymethyl cellulose (CMC) acting as an anionic adsorbent and cellulose nanofibrils (CNFs) as strengthening filler, both supported with simultaneous citric acid mediated cross-linking. The effects of operating parameters (filtration pressure and velocity, feed concentration) on the performance of dead-end filtration in terms of permeate flux (flow rate) as well as the adsorption (binding capacity) of Lysozyme/Lys as a model protein, will be shown. In addition, the Lys binding capacity and permeability/recovery, evaluated by a chromatographic mode, will be presented, showing their potential for biochromatography.

Structural and surface properties of CMC-CNF based membranes for purification and chromatographic separation of lysozyme as a model biopharma protein

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.