Corneal epithelia cells in the eye are guided by the ordered fibre arrays and a naturally occurring DC electric field (EF).But little is known about the mechanisms and guidances of cell migration by nanotopography or by an EF.Here,we used bacterial cellulose(BC) , which is one of the most common fibrous materials in nature,to mimic similar ordered fiber alignment with natural tissue by stretching.The tensile strength of the unstretched BC/Gelatin film is 137.95MPa and it can be enhanced from 286.26 to 832.32Mpa as the strain was increased from 20% to 40%.And we examined cell migration on stretched BC/Gelatin(40%) with a physiological EF(150mv/mm) separately and in combination.Corneal epithelia cells aligned parallel to the fiber alignment and the migration speed of cells on stretched BC/Gelatin were enhanced compared to cells on unstretched BC/Gelatin.When the direction of an EF was parallel to fiber alignment, cathodal electrotaxis along fiber alignment was enhanced compared to that on unstretched BC/Gelatin.Therefore, the migration of corneal epithelia cells can be enhanced by co-operative of stretched BC/Gelatin and DC electric fields,which can be exploited in tissue engineering.

Guidance of corneal epithelial cells migration by co-operative effects of stretched bacterial cellulose/gelatin and DC electric fields

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.