A strain of yeast which can monodemethylate Nobiletin on the A ring was separated form Chenpi (old citrus peel). ITS results showed it is a mutant specie of Filobasidium magnum with 4 base-pairs less than the documented one. In order to improve the demethylation efficiency, Nobiletin was encapsulated in modified starch by milling process. 5 hours milling processing produced starch-encapsulated-Nobiletin solution with particle size being around 900 nm. Lyophilized powder was applied for fermentation. The effect on demethylation efficiency of starch-encapsulated-Nobiletin concentration was firstly tested with results showing the optimum concentration being at 15μg/ml media. The yield of the two monodemethylate Nobiletin could respectively reach 40 μg/ml and 10 μg/ml after 14 days of fermentation, which was 20 times higher as compared to that when Nobiletin was dissolved in DMSO, and 5 times higher than that of Nobiletin-starch water solution without milling process. Encapsulation of Nobiletin in modified starch by milling process is an efficient method to boost the transformation efficiency of insoluble chemical compounds.

Improved demethylation efficienty of nobiletin encapsulated in modified starch by miling process

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.