This presentation will highlight findings from a study using new multimodal membranes for high throughput polishing at elevated solution conductivities. Multimodal membranes combine Coulombic interactions, hydrophobic interactions and hydrogen bonding in binding bioprocess impurities, thereby enabling salt-tolerant performance under conditions where conventional ion-exchange media fail. In this study, we evaluated the performance of Purilogics’ multimodal anion-exchange membranes (PurexaTM-MQ) under a variety of industry relevant conditions. We will present dynamic binding capacity (DBC) measurements of bovine serum albumin (BSA), human immunoglobulin G (hIgG), and salmon sperm DNA (ssDNA) for a range of conductivities (0 -30 mS/cm), salt types, flowrates, and pH values (6-9). Using 1 mg/ml BSA, measured DBC values were greater than 90 mg/ml for the highest conductivities but showed a maximum of 130 mg/ml at around 6 mS/cm. DBC increased by 42% with a three-fold increase in BSA concentration. DBC values showed only 5% decrease over 40 cycles with regeneration. The membranes had exceptionally high ssDNA DBC of 50 mg/ml under high conductivity conditions, easily outperforming all other commercial products. Purilogics’ PurexaTM-MQ membranes can rapidly purify bioprocess pools without dilution or desalting of feed, and may also hold potential applications for DNA production in the gene therapy industry.

New multimodal anion-exchange membranes for polishing of biologics

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.