Many of today’s bioprocesses require flexible manufacturing strategies in order to maintain a diverse multiproduct facility. Membrane adsorber chromatography is an effective tool to improve manufacturing flexibility by enabling rapid mass transfer and eliminating the need for column packing. This technology also offers opportunities for increased purification productivity as compared to resin based technologies. While membrane adsorbers provide value as a single-use device, in some applications the ability to regenerate the device for multiple re-use cycles may be desired. In this work, a commercially available high capacity membrane adsorber featuring quaternary amine chemistry was evaluated for re-use feasibility in a monoclonal antibody polishing application. Results are presented to evaluate device performance over consecutive processing cycles with a focus on product yield, host cell protein purification, viral clearance, and device backpressure. Considerations for implementing membrane adsorber chromatography in both single-use and re-use applications are discussed.

Evaluating cleaning and re-use feasibility for an anion exchange membrane adsorber

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.