Sanofi’s continuous biomanufacturing platform affords numerous advantages over both legacy perfusion processes and traditional fed-batch systems. Process intensification allows for an order of magnitude higher cell density resulting in increased volumetric productivity. Intensified processes rely heavily on automation with fewer manual operator inputs in order to ensure robust operations.<br/><br/>Dissolved oxygen probes in intensified high density cell culture processes are plagued by gas bubble adherence related noise. Excessive noise may lead to suboptimal oxygen supply resulting in hypoxic cell culture conditions that impact growth and product quality. A root cause analysis was performed to identify factors such as raw materials, equipment, and automation strategies that might influence ability to control dissolved oxygen.  We propose a strategy to address common problems observed in dissolved oxygen control and present the proof of concept obtained in an at-scale production vessel. <br/>

Augmenting dissolved oxygen control in intensified cell culture processes

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.