Advances in bioreactor technologies over the last decade have increased productivity and expression to levels where current capabilities of protein A resins are challenged.  To study ways to increase capture productivity, reduced scale comparative evaluations of five different Protein A capture resins and 3 types of Protein A membranes for capturing monoclonal antibodies were performed. Dynamic binding capacity (DBC) was measured for each resin and membrane across a range of flow rates and fitted to hyperbolic functions. Resulting data were used to model productivity in various operational modes. Resin lifetime was estimated by measuring DBC after timed exposure to sodium hydroxide. Results of the analysis were used along with target process parameters to calculate expected operating costs for each resin type in various scenarios representing high volume commercial processing and low volume or infrequent processing, representing a clinical development scenario.

Comparison of productivities and cost models for protein A capture matrices

Macromolecular Chemistry: The Second Century

[Author Benefits](https://scimeetings.acs.org/?utm_source=pubs_content_marketing&utm_medium=website&utm_campaign=0320_MCF_NPI_Launch_Spring_Homepage&ref=pubs_content_marketing)

[How to use SciMeetings](https://storage.googleapis.com/dl.morressier.com/acs/SciMeetings-ACSSpring2020-HowToGuide_M3020-Final.pdf)

[Frequently Asked Questions](https://storage.googleapis.com/dl.morressier.com/acs/SciMtng_ACSNMSp2020_FAQ_M3120_Final.pdf)

ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.