Commingled contamination of trichloroethene (TCE) and 1,4-dioxane (dioxane) has been perceived as one of the most prevalent groundwater remediation challenges. Considering their risks to human health and natural biota, an effective and cost-efficient treatment for <i>in situ</i> remediation is desired. In this study, we developed a biotreatment train that integrates anaerobic dehalogenation of TCE by SDC-9 and aerobic cometabolism of dioxane using a propanotrophic isolate <i>Azoarcus</i> sp. DD4. Microcosms were prepared with groundwater and bedrock samples from a TCE and dioxane impacted site located in New Jersey. All TCE (~20 µM) underwent a stepwise dehalogenation by SDC-9 anaerobically within 4 weeks to cDCE, then completely converted to VC after 16 weeks. After air exposure, the system was then converted into aerobic condition (DO &gt; 8.00 mg/L and ORP &gt; 50 mV). The subsequent aerobic microcosm demonstrated that dioxane can be completely removed by DD4 with propane from the initial 20 µg/L to below our method detection limit (0.38 µg/L) in 4 weeks. Even better, DD4 simultaneously co-oxidized cDCE and VC accumulated during the previous anaerobic treatment, although the presence of these cVOCs delayed the degradation of dioxane. Pure culture studies further revealed cDCE has a greater inhibitory effect to DD4 than VC. Overall, anaerobic SDC-9 bioaugmentation followed by aerobic treatment with DD4 exhibited high efficiency and feasibility to clean up the mixed contamination of TCE and dioxane.

Sequential anaerobic and aerobic bioremediation of commingled contamination of trichloroethene and 1,4-dioxane

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.