Aptamers are single-stranded DNA or RNA oligonucleotides that bind to a molecular target with both high affinity and selectivity due to their distinct three-dimensional structures.  Target-specific aptamers are mostly screened from a random DNA or RNA pool using a technique called Systematic Evolution of Ligands by EXponential enrichment (SELEX).  A standard nucleic acid library contains a randomized central region and a fixed primer region at each end for amplifying target-bound central sequences via PCR.  The forward and reverse primer sequences can cause non-specific binding of the target to these regions, and/or can interfere with target binding to the central region by their own interactions with the central sequences.  The author devised a new type of oligonucleotide library designed so that the fixed primer sequences (~20 nucleotides) at each end of the library’s fragments would not disrupt the target’s binding to the randomized central region (~40 nucleotides) of each fragment.  The modified library design provides more flexibility for developing an optimized aptamer sequence with high affinity for, and selective binding to, a particular target.  <br/>

New type of oligonucleotide library for SELEX aptamer selection

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.