Type II polyketides are polyaromatic bacterial secondary metabolites which have seen great success in the clinic.  Doxorubicin and tetracycline are two such FDA-approved drugs which showcase the potential anticancer and antibiotic properties of type II polyketides.  These molecules are produced via enzymatic assembly lines (polyketide synthases; PKSs) encoded by biosynthetic gene clusters. Further study of diverse type II PKSs can allow for a greater understanding into how enzymes in the biosynthetic pathway interact to form structurally complex and pharmacologically relevant molecules. Our research focuses on the in vitro characterization of key enzymes in the type II PKSs encoded by ancient and orphaned biosynthetic gene clusters.  We have been able to heterologously express several of these key enzymes from various previously-unstudied bacterial strains.  We have also characterized and confirmed the expression of these enzymes via various analytical techniques including gel electrophoresis, sedimentation velocity analytical ultracentrifugation, and fast protein liquid chromatography.  These methods of characterization will allow for novel insights into the in vitro structure of these type II PKS enzymes.  By harnessing the powerful biosynthetic capability of type II PKSs and studying how these enzymes interact with their native partners, we can develop tools to attain new chemical diversity through chemical engineering approaches.

Heterologous expression and characterization of ancient orphaned Type II polyketide synthase enzymes

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.