The adeno-associated viral vector (AAV) has been broadly used as a delivery tool for gene therapy applications.  Development of a robust purification process is essential in delivering high purity and potency AAV products to the clinic.  The short clinical timelines and limitation of materials to support process development pose unique challenges when developing robust and scalable downstream purification processes.<br/><br/>Our approach in overcoming this challenge is to leverage high throughput (HTP) technology for purification process development.  In this study, we demonstrated the capability of the TECAN automated liquid handling system to develop purification processes using robocolumns and self-packed resin plates.  The purification performance and product quality achieved in the high throughput format was compared with that obtained in the bench scale column chromatography.  The established high throughput strategies demonstrated good feasibility, significantly reduced material needs, and reduced development timelines. With further expansion and improvement, we envision that this HTP platform can be used for optimization and characterization of downstream purification processes used for manufacturing of gene therapy products.<br/>

Leveraging high-throughput strategies to accelerate AAV purification process development

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.