This lecture will describe the use of non-canonical amino acids (ncAAs) as selective probes of protein synthesis in complex biological systems.  Pulse-labeling with ncAA probes provides time-resolution, while controlled expression of mutant aminoacyl-tRNA synthetases allows the investigator to restrict analysis to cell types or cell states of interest.  The methods are applicable to studies of microbial systems, mammalian cell culture, and a variety of animal models.  The scope and limitations of the approach, and some of our most recent results, will be discussed.<br/>

Selective proteomic analysis of cellular sub-populations in complex biological systems

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.