Therapeutic Recombinant proteins are exposed to different kinds of stress conditions during production, storage, and shipping that can result in the formation of soluble and insoluble aggregates. The potential for intermolecular protein-protein interaction and subsequent increased probability of aggregation is observed at air-water interface where protein chains orient themselves to expose their hydrophobic pockets to increase their interaction with the aqueous surface. Non ionic surfactants like Polysorbate 80 and 20 are widely used in protein formulations to prevent agitation-induced aggregation because of its effectiveness at low concentrations, relatively low toxicity, ability to inhibit protein surface adsorption, aggregation under various processing conditions and act as a stabilizer against protein aggregation. Nevertheless, the alkyl polyoxyethylene side chains of Polysorbates undergo auto oxidation resulting in formation of hydroperoxides, side-chain cleavage, and eventual formation of short-chain acids, such as formic acid, which can affect the stability of the product. In this work, we have evaluated various biosurfactants and investigated their interfacial properties after having compared their performances with traditionally used Polysorbates for transdermal drug delivery application. Preliminary data suggests that the microbially derived Biosurfactants could be a better alternative of Polysorbates for proteins stabilization at the air-water interface over a diverse range of physicochemical properties, such as hydrophobicities, pKa’s and degrees of ionisation. This effect was attributed to the formation of a recombinant protein-biosurfactant interaction which influenced the diffusion profiles of the drug molecules.

Interfacial study of biosurfactants in recombinant protein formulation for transdermal drug delivery applications

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.