Gold nanoparticles (AuNPs) have remained a research topic because of their extensive range of applications which includes biosensing, cancer therapy, and drug delivery. In biological applications one of the most pertinent interactions is the corona formation (proteins/NPs complex). This project focused on studying the interactions of human serum albumin (HSA), the most abundant protein in human blood, with AuNPs using fluorescence correlation spectroscopy (FCS). Atto655 was used as a probe, because its absorption and emission are in the red region, away from AuNP plasmonic absorption. We proposed that through FCS detailed information could be obtained about the interaction of HSA with AuNP and how these interactions change with NPs’ surface modification at the single molecule level. Results demonstrate that the emission of Atto655, i.e. the average number of molecules (&lt;N&gt;) and the brightness, decreases in the presence of β-cyclodextrin-AuNP (bCDAuNP) and Citrate-AuNP (cAuNP). Addition of HSA (0-100nM) to Atto655:bCDAuNP induces a recovery of free &lt;N&gt;, while for Atto655:cAuNP remains low. When HSA is added to polyethylene glycol (PEG) modified AuNPs, &lt;N&gt; remains constant. Thus, the information gathered at single molecule level provides a better understanding of the binding mechanism of macromolecules to AuNPs.

Interactions of β-cyclodextrin modified gold nanoparticles with human serum albumin at single molecule level

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.