Reactive carbonyl species including methylglyoxal (MGO) are oxidation metabolites of glucose and precursors of advanced glycation end products (AGEs). They mediate cellular oxidative stress and could exacerbate skin complications including those related to diabetes mellitus. Published data support that phytochemicals, including phenolic compounds, can exert cellular protective effects by their antioxidant and MGO-scavenging activity. A phenolic-enriched maple syrup extract (MSX) was reported to show inhibitory effects against MGO-induced formation of AGEs and protective effects against AGEs- and MGO-induced cytotoxicity in human colon cells but its skin protective effects remain unknown. The protective effects of MSX were evaluated against hydrogen peroxide (H2O2)- and MGO-induced cytotoxicity in human keratinocytes (HaCaT cells). Cellular viability was evaluated for the ameliorative effects of MSX against H2O2- and MGO-induced cell damage using the CTG assay. Cellular antioxidant effects of MSX were evaluated in the reactive oxygen species (ROS) assay. In addition, the cellular DNA integrity of HaCaT cells was evaluated by the comet assay. MSX (at 50 µg/mL) markedly reduced H2O2- and MGO-induced ROS production in HaCaT cells by 64.2% and 50.7%, respectively. In addition, MSX (at 50 µg/mL) reduced MGO-induced DNA damage in HaCaT cells. Conclusion: MSX showed protective effects against H2O2- and MGO-induced cytotoxicity in HaCaT cells supporting its potential for dermatological applications.

Phenolic-enriched maple syrup extract reduces oxidative stress in human keratinocytes HaCaT cells

Macromolecular Chemistry: The Second Century

[Author Benefits](https://scimeetings.acs.org/?utm_source=pubs_content_marketing&utm_medium=website&utm_campaign=0320_MCF_NPI_Launch_Spring_Homepage&ref=pubs_content_marketing)

[How to use SciMeetings](https://storage.googleapis.com/dl.morressier.com/acs/SciMeetings-ACSSpring2020-HowToGuide_M3020-Final.pdf)

[Frequently Asked Questions](https://storage.googleapis.com/dl.morressier.com/acs/SciMtng_ACSNMSp2020_FAQ_M3120_Final.pdf)

ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.