The 5-cyanopyrimidine based compounds can exhibit cytotoxic activity against the A431 cell line. To evaluate this the hit compounds SV296 and SV350, we studied the induction of cell death and apoptosis using flow cytometry. A431 cells that were treated with IC50 concentrations of SV296 demonstrated a slight decrease in in the number of living cells to 91.52% contrary to 96.86%. SV350 compound causes a decrease in the number of living cells to 86.92%, that which is comparable to gefitinib activity (85.47% of living cells).<br/>In order to examine whether inhibition of SV296 and SV350 was mediated by induction of cell cycle arrest the effect of SV296 and SV350 treatment on cell cycle distribution was analyzed by flow cytometry. As shown treatment of cells with 3.9 µM of SV296 and 6.4 µM of SV350 for 24 h resulted in G0/G1 phase accumulation of cells (69.56% and 63.60% contrary to 48.51 in control cells. SV296 and SV350 treatment induced a significant accumulation of cells in the G0/G1 phase with a parallel depletion of the percentage of cells in G2/M and S phases.

New 5-cyanopyrimidine based compounds cause apoptosis and cell cycle arrest in a431 tumor cells

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.