Natural Products are secondary metabolites that can be extracted and isolated from any living organism. Due to their chemical diversity and biochemical relevance, the identification of bioactive compounds is of great interest to the pharmaceutical industry for their potential use towards the development of new medicine especially those that are cytotoxic to cancer cells. The brine shrimp (<i>Artemia</i> spp.) lethality assay is an appropriate preliminary screening of cytotoxic compounds since the brine shrimp’s lethality correlates to the cytotoxicity of human cancer cells from the lung and colon lining. The aim of this work is to isolate and identify cytotoxic bioactive compounds for their potential use in pharmacology from fractionating active marine invertebrate extracts. The evaluated extracts are obtained from the University of Oklahoma Schmitz Sponge collection. Each extract is screened utilizing the brine shrimp assay at a low, medium, and high concentration (10.0 ppm, 50.0 ppm, and 100 ppm). Extracts indicating 70% lethality are considered active and will be fractioned by normal and reverse phase flash chromatography and their activity tested using the brine shrimp lethality assay. If fractions are still bioactive after reverse phase flash chromatography, they will be analyzed using nuclear magnetic resonance (NMR) spectroscopy, mass spectrometry, and infrared (IR) spectroscopy to elucidate the structure of the bioactive compound.

Isolating bioactive compounds from marine invertebrates

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.