Glioblastoma tumors (GBM) are the most deadly of the brain of an adult tumor. EGFR signaling impairment is the main characteristic of the classic GBM subtype, and EGFR signaling has a main role in the progression of a GBM tumor. Cellular spheroids are one of these 3D models where clusters of glioblastoma cells will undergo self-assembly with the formation of three-dimensional tumor-like structures.<br/>U87MG cells (5,000) were seeded in 3D Matrigel culture. After 7 days, the cultures were treated with DMSO or EGFR inhibitors (gefitinib and 5-cyanopyrimidine compounds – SV296 and SV350 at concentration 6.8 μM and 3.9 μM). Fresh growth medium containing DMSO or was replaced every 3 days for 14 days, and, after staining with DAPI and confocal images were obtained. Scale bar, 60 μm. Alternatively, U87MG spheroids were treated with SV296, SV350 or gefitinib for 14 days and morphology of 3d cell cultures was measured.<br/>According to the data obtained, introducing 5-cyanopyrimidine derivatives SV296 or SV350 into the cell culture causes a significant decrease in the size of the formed monocellular spheroids from U87MG cells. Gefitinib caused a less pronounced decrease in size (Fig. 1A). When SV296 and SV350 were introduced into the culture of the formed spheroids and cultivation, a violation of the spheroid structure for both compounds occurred. Gefitinib caused less structural disturbance (Fig.1 B).

5-cyanopyrimidine based compounds modulate the U87MG glioblastoma spheroid cultures morphology 

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.