Chronic neuropathic pain is a common debilitating affliction with a myriad of causes and treatments. Clinical evidence indicates that the application of electrical stimulation (ES) to neural tissue provides efficacious pain relief, although molecular mechanisms by which the therapeutic effects arise are not fully understood and in need of description. A contributing factor to the development and maintenance of chronic neuropathic pain can be ascribed to oxidative stress processes mediated by astrocytes in the nervous system. It is hypothesized that this particular population of glia plays its role in neuropathic pain via production of reactive oxygen species (ROS) and reactive nitrogen species (RNS). Development of a model cell system through which radical species can be reproducibly measured is necessary to understand how astroglia produce ROS/RNS in response to ES and other environmental conditions. Incubation of C6 glioma cells with a fluorescent probe for superoxide or nitric oxide has proven useful in assessing theses intracellular species in response to chemical perturbates, such as the gliotransmitters glutamate and adenosine. Preliminary data support that some gliotransmitters influence superoxide and nitric oxide production. Pending work includes obtaining subsequent reproducible, quantitative measures via a fluorometer for both superoxide and nitric oxide to compare relative changes between these reactive radical species in experimental environments. Future work includes the use of a calcium probe to query the relations between calcium mobilization and these reactive radical species.

Reactive oxygen (ROS) and reactive nitrogen species (RNS) detection in axenic C6 glioma cells: Assessment of the role of electrical stimulation

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.