The National Oceanic and Atmospheric Administration’s long-established Mussel Watch Program (MWP) uses freshwater mussel, <i>Dreissena spp</i>, as a bioindicator species to track the impact of pollutants on coastal ecosystems in the Great Lakes. Recently, metabolomics analysis has been used to evaluate the physiological response of mussels inhabiting areas of concern. In these studies, metabolomic analyses were applied to the polar extracts of mussel. In an effort to understand the biochemical effect of pollution more broadly, an investigation was made into lipid changes using nuclear magnetic resonance (NMR). The objective of this study was to determine how the mussel lipid profile varies based on collection season, location and handling method (caged vs <i>in situ</i>). To represent the given parameters, six sets of ten mussels were collected from two different sites in Lake Michigan (n=60), north Milwaukee harbor (LM-04) and one reference site (LM-05). Collected mussels were lyophilized whole and in-shell, individually homogenized and extracted with chloroform/methanol/water. The lipid profile was measured by 1H NMR. Principle component analysis (PCA) of the NMR spectra found no distinguishable difference between site, time of collection or handling method, similar to the findings of a previous study at these sites assessing the polar metabolome. However, univariate analysis of the spectra revealed triacylglyceride, diacylglyceride, phosphatidylcholine, and phosphoenolamine glycerol protons, in addition to an unknown peak, varied between sampling sites and time of collection. These preliminary results suggest NMR-based lipidomics can provide lipid class information as an ecotoxicological tool to understanding health and help guide future targeted investigations into the biochemical impact of areas of concern.

NMR-lipidomics measurement of Great Lakes mussels as an ecotoxicological assessment of areas of concern

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.