Nano-biosensor technology based on surface enhanced Raman scattering (SERS) is a potential sensitive and reliable method for early detection of tumors. The unique morphology, template structure, modifiability and biocompatibility of cellulose nanocrystals (CNC) make them excellent supporters for constructing multifunctional SERS nanocomposite biosensors. Carboxylated cellulose nanocrystals (CNC-COOH) were converted to thiolated cellulose nanocrystals (CNC-SH) via EDC/NHS reaction. CNC-SH with mercapto contents in the range of 0.05 - 0.2 mmol/g were obtained by adjusting the dosage of cysteamine. SERS nano-biosensors of CNC loaded with Au nanospheres (AuNSs@CNC) were constructed by both self-assembly and <i>in-situ</i> synthesis methods. Uniform AuNSs with the sizes of 10, 30 and 60 nm were deposited on the surface of CNC-SH via Au-S and the loading density and spacing were further regulated by mercapto contents. For the <i>in-situ</i> synthesis method, the loading of AuNSs on CNC was also controlled by changing the mercapto contents of CNC-SH and the growth conditions. The results show that the size and spacing of AuNSs on CNC determine the SERS sensitivity of the composite substrate. With the decrease of gap distance between the loaded AuNSs, the SERS signal increases gradually. The enhanced SERS sensitivity can be ascribed to the formation of hot spots. The optimum size of AuNSs is about 30 nm. This type SERS probe based on AuNSs@CNC nanocomposite shows potential applications in early diagnosis and treatment of cancer tissue.

Preparation of Au nanoparticles/cellulose nanocrystals composites and their SERS performance

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.