Globally, there is an alarming and persistent rise in the number of people affected by the Type II diabetes (T2DM) without a commensurate increase in the amount of benign natural curative remedies available to assist in tackling this issue. This necessitated this work aimed at identifying potent naturally occurring anti-diabetes compounds from Chromolaena odorata. The crude methanolic leaves extract of the sample was subjected to gradient elution column chromatography from 100% hexane to 100% ethyl acetate. The bioassay guided studies of the fractions resulted in the isolation of three known flavonoid compounds but for the first time from C.Odorata tagged Siam 1, 2 and 3. Using the combination of 1D and 2D Nuclear Magnetic Resonance (NMR), UltraViolet, InfraRed, Mass spectrometry experimental data, and relevant literatures, the structures of the isolated compounds were elucidated. Nine analogues of siam 2 were equally made using the modified Williamson ether synthesis. In vitro bioassay studies of the crude fraction, isolated compounds and the synthesized analogues against yeast alpha-glucosidase comparatively with acarbose was done using the micro plate reader. Molecular docking studies of the most active compounds were done using the Autodock vina of the Scripps Research Institutes. IC50 values obtained showed that acarbose (0.4417 ±0.071mg/mL) &gt; siam 1 (0.999±0.068 mg/mL) &gt; siam 3 (2.09±0.647 mg/mL) &gt; siam 2 (2.385±0.128mg/mL) &gt; crude C.odorata (4.039±1.43 mg/mL). Bioassay studies of the analogues at 1.25mg/mL showed elevated activity with increase in chain length, branching and unsaturation. Results obtained from the molecular docking studies showed that the non-competitive activity of these compounds were based on their hydrogen bonding interaction with amino acid residues (Isoleucine 362 and Valine 446) in the crystal structure of yeast alpha-glucosidase enzyme. Findings from this study suggest that flavonoids and their alkyl analogues derived from C.odorata have the potential to be developed into useful drugs for the management of T2DM.

Isolation, derivatization, alpha glucosidase assay and docking studies of anti-diabetes metabolites from leaves of Chromolaena odorata (Siam weed)

Macromolecular Chemistry: The Second Century

[Author Benefits](https://scimeetings.acs.org/?utm_source=pubs_content_marketing&utm_medium=website&utm_campaign=0320_MCF_NPI_Launch_Spring_Homepage&ref=pubs_content_marketing)

[How to use SciMeetings](https://storage.googleapis.com/dl.morressier.com/acs/SciMeetings-ACSSpring2020-HowToGuide_M3020-Final.pdf)

[Frequently Asked Questions](https://storage.googleapis.com/dl.morressier.com/acs/SciMtng_ACSNMSp2020_FAQ_M3120_Final.pdf)

ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.