Nanocarrier-mediated protein delivery is a promising strategy for the treatment and diagnosis of human disease. However, most delivery systems are limited by endosomal entrapment of delivered protein cargo. Herein we report a robust, versatile polymeric-protein nanocomposite (PPNC) platform to achieve cytosolic protein delivery under biologically relevant conditions. We synthesized a library of “semi-rigid” polymers with varying molecular weights and cationic functionality to stabilize and deliver co-engineered proteins. This platform was capable of cytosolic protein delivery as evaluated by a versatile flow imaging cytometric method. Higher molecular weight polymers demonstrate a larger range of effective formulations for protein delivery under serum conditions, making them robust agents for therapeutic protein delivery with potential for clinical translatability.

Rationally designed polymeric vehicles for versatile cytosolic protein delivery

Macromolecular Chemistry: The Second Century

[Author Benefits](https://scimeetings.acs.org/?utm_source=pubs_content_marketing&utm_medium=website&utm_campaign=0320_MCF_NPI_Launch_Spring_Homepage&ref=pubs_content_marketing)

[How to use SciMeetings](https://storage.googleapis.com/dl.morressier.com/acs/SciMeetings-ACSSpring2020-HowToGuide_M3020-Final.pdf)

[Frequently Asked Questions](https://storage.googleapis.com/dl.morressier.com/acs/SciMtng_ACSNMSp2020_FAQ_M3120_Final.pdf)

ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.