Cell heterogeneity leads to differences in tumor proliferative capacity, invasive ability and drug sensitivity. The expression and time-space distribution of membrane protein are closely related to changes in cell behaviour and pathology. Thus, precise quantification of the membrane protein expression level at single cell level is crucial for early cancer diagnosis and efficient treatment. Thanks to the accurate molecular formula of peptide-cluster probe and laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) technique, single cell quantification of membrane proteins expression level could be achieved. Next, for the purpose of assessing the risk of primary lung tumor invasion/metastasis, we constructed peptide-cluster probe and quantified membrane type-1 matrix metalloproteinase (MT1-MMP) levels in primary tumor tissue via laser ablation inductively coupled plasma mass spectrometry 2D mapping (2D-LA-Mass Mapping), which will provide a precise assessment of tumor regional lymph node invasion and remote organ metastasis.<br/>

Peptide−cluster probe based single-cell quantification of tumor cell membrane proteins

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.