Mutation in p53 sequence, a cancer related gene, has been implicated for more than 50% of human cancer, thus selective and sensitive detection of p53 gene is of great significance in the early-stage diagnosis and targeted therapy of cancer. However, most of the ultrasensitive detection strategies reported so far either involves complex design or requires multi-step signal amplification. To address these challenges, we have designed a single-step FRET-based sensor that allows a sensitive detection of p53 by single-molecule counting. In addition to its simple design, this sensor allows zero-background and thus the detection limit is down to low femtomolar (fM) concentration without the need for target amplification. We also observed that this sensor is highly effective in discriminating single nucleotide polymorphisms (SNPs). Since simultaneous detection of different p53 mutants could be useful for screening genetic disorders, we are currently investigating the multiplexing ability of our sensor in order to simultaneously detect and quantify multiple targets.

Single molecule detection of a cancer susceptible gene

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.