In recent years, simultaneous detection of multiple biomarkers has drawn much interest in the fields of bionanotechnology and clinical diagnostics. Recent studies have found a myriad of disease-related biomarkers spanning a wide variety of biomolecules and it has been shown that the accuracy of diagnosis increases significantly by measuring the level of multiple biomarkers instead of just one. Current popular methods of multiplexing, including microarrays, multiplexed polymerase chain reaction (PCR), and DNA barcodes often suffer from complex engineering and may yield only semi-quantitative information. Although single molecule fluorescence resonance energy transfer (smFRET) is gaining popularity in multiplexed sensing as it can provide a sensitive and quantitative platform to tackle these limitations, it often requires complex labeling schemes and multicolor excitation sources. We have recently reported a multiplexed detection of three different DNA sequences using the toehold-mediated strand displacement process. This method requires only a single FRET pair for multiplexing, however, the limit of detection is ~200 pM. To expand the application of the method to detect micro RNA biomarkers that are present at low femtomolar (fM) concentration in biological samples, we have re-imagined our sensor design so that it does not require a toehold displacement process and enables a lower detection limit. Overall, we anticipate that our multiplexed method may find applications for high-confidence detection of biomarkers.

Distance tunable multiplexed sensing: Single molecule FRET approach

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.