Agave syrup has gained popularity as an alternative to traditional sweeteners due to its low glycemic index. Knowledge of the chemical composition of foods is important not only for human health but also for authenticity. One method to detect the possible adulteration of agave syrup is by looking for changes in its major carbohydrates (fructose and glucose) and its oligosaccharide profile using high performance anions exchange chromatography with pulsed amperometric detection (HPAE-PAD).<br/><br/>A new operating mode for HPAE-PAD systems, Dual Eluent Generation Cartridge (Dual EGC) mode, is available. This mode of operation replaces the manual preparation of the sodium hydroxide/sodium acetate eluents. This mode uses a methanesulfonic acid (MSA) EGC cartridge and a potassium hydroxide (KOH) EGC cartridge in series to generate the extremely reproducible and accurate KOH/KMSA eluent gradient needed for separating complex carbohydrates.<br/><br/>A Dual EGC gradient method was developed for carbohydrate analysis in agave syrup. This method was used to determine inositol, sorbitol, mannitol, HMF, glucose, fructose, and sucrose. Carbohydrate profiles before and after amyloglucosidase and fructanase enzyme treatment are compared. Comparison with a traditional HPAE-PAD separation of agave syrup using sodium hydroxide/sodium acetate eluents showed that the Dual EGC method delivers similar resolution of agave carbohydrates, but simplifies operation (no eluent preparation) and improves the precision and accuracy.

Carbohydrate analysis of agave syrup using HPAE-PAD in dual eluent generation cartridge mode

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.