Textile dye contamination is a serious problem that reduces soil productivity by destabilizing microbial community structures. Here, we investigated the influence of bioaugmentation on the degradation of a mixture of dyes (MOD) and textile industry effluent (TIE) in soil microcosms using eight different dye-degrading bacteria. The biodegradation potential improved in the bioaugmented microcosms, especially in the initial phase. The bioaugmented MOD and TIE microcosms exhibited 98.33% and 94.19% decolorization and 96.92% and 95% chemical oxygen demand (COD) removal, respectively, at day 30. Several dye-catalyzing enzymes were examined, and the activities of lignin peroxidase, veratryl alcohol oxidase, tyrosinase, and azoreductase were found to be significantly induced (&gt;three-fold increase) in bioaugmented microcosms due to the addition of textile dyes and bacteria. Changes in alpha diversity induced significant alterations in the microbial community structure of the microcosms because of continuous MOD and TIE flow. The Rheinheimera, Kocuria, Ruminococcaceae UCG-010, Ralstonia, and Pseudomonas bacterial assemblages were found to be dominant after exposure to MOD and TIE, indicating that these genera are key in dye biodegradation. The bacteria used for augmentation, namely, Staphylococcus, Bacillus, Arthrobacter, and Pseudomonas, survived well in the soil microcosms and their relative populations increased, suggesting that they co-existed synergistically with the indigenous microflora. Functional annotation of metagenomes illustrated that xenobiotic pathways such as benzoate, aminobenzoate, chloroalkane and chloroalkene, etc. degradation may provide alternate pathways for MOD and TIE detoxification. Our results indicate that augmented dye degrading bacteria and soil microbiome have established a potentially mutualistic symbiotic relationship with increase in detoxification rate leading to sustainable approach for restoration of contaminated agricultural sites.

Metagenomics study of soil microcosms to evaluate the bioremediation of textile dyes in a contaminated ecosystem

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.