Yearly outbreak of influenza causes millions of cases of infections worldwide, with about ten percent being fatal. The pandemic risk of the disease and the high mutation rate of the viruses have urged scientists to continuously and rapidly develop new kinds of vaccines with higher qualities. In this work, we demonstrate the development of vaccine production process against the Influenza A H1N1 subtype, using recently developed ion exchange resins. The aim is to develop a low cost process while maintaining high quality and safety of the product.<br/><br/>H1N1 virus was propagated in serum free medium using Vero cells as a host and released into the culture supernatant. The supernatant was separated by centrifugation, heat-treated for virus inactivation, followed by Benzonase treatment and concentration by TFF. H1N1 virus concentrate was then captured on a wide pore anion exchanger and subsequently polished by wide pore cation-exchanger. High purity and good overall yields were achieved with this purification. Digital-droplet PCR (ddPCR) with high sensitivity was used as the main quantitative assay throughout the whole process. All purification steps used here are scalable and can be adapted for large-scale production, making the process is an efficient alternative for Influenza A H1N1 vaccine production.

Two-step purification process for H1N1 virus using ion exchange chromatography resins

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.