Nanoparticles have become an integral part of our modern society, where we can find them in fuel cells, advanced batteries, consumer electronics, and biomedical field. However, the environmental impacts of disposed nanomaterials are yet to be better understood. This research focuses on studying a gram-negative Ralstonia sp., a bacterium isolated from a highly toxic polycationic nanodiamond particle solution and understanding its resistance mechanism by comparing it to a model gram-negative bacterium, Shewanella oneidensis. We have previously identified the toxicity mechanism by cationic polymer-capped nanoparticles is by bacterial membrane damage. Therefore, we are aiming to understand the membrane interactions of poly-allyl amine hydrochloride covalently-bound diamond nanoparticles (PAH-DNPs) and cell membrane of Ralstonia sp.. To assess and compare the toxicity and the extent of membrane damage induced by PAH-DNPs and PAH ligand, we used a Growth-based Viability (GBV) assay and a Live/Dead assay on Ralstonia sp. and Shewanella oneidensis. The result from GBV assay suggests that Ralstonia sp. is more susceptible under low concertation of PAH-DNPs than Shewanella oneidensis, but more viable at higher concentrations of PAH-DNPs. Moreover, Live/dead assay result suggested that Ralstonia sp shows more severe membrane damage than Shewanella oneidensis. Interestingly, at the same amine-concentrations, PAH ligand alone is much less toxic to both bacterial strains. We are currently building bacterial model membranes to understand the molecular interactions between PAH-DNPs and bacterial surfaces.

Investigation of bacterial surface properties towards their resistance to toxicity by cationic nanoparticles

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.