In recent years, the use of nanotechnology has dramatically increased in a variety of industries due to the antimicrobial and antibacterial properties of the nanomaterials. Because of their widespread commercial use, there is an unprecedented level of silver nanoparticles (AgNPs) leaking into the water systems, with unknown implications and effects on the environment and its diverse inhabitants. For this reason, it is crucial to understand how AgNPs interact with various environmental matrices, for example, with proteins. UV-Vis spectroscopy, dynamic light scattering (DLS), and capillary electrophoresis (CE) assays were developed and applied to understand these complicated systems. The localized surface plasmon resonance (LSPR) of AgNPs can be monitored by UV-vis and shifts in its wavelength maximum caused by adsorption of bio-molecules can be used to quantify binding kinetics and affinity using Langmuir adsorption isotherms. Similarly, DLS measurements can be used as secondary confirmation of binding interactions by evaluating how adsorption affects particle diameter. Finally, CE can be used to separate protein-bound from unbound AgNPs and binding and rate constants can be extracted from the separation data. These techniques were applied to quantify the protein coating, or “corona”, formed on AgNPs. Three model proteins were examined including bovine serum albumin (BSA), human serum albumin (HSA), and glycated HSA (g-HSA). BSA is an affordable model protein that was used for optimization of each assay. The binding characteristics of HSA and g-HSA were then compared to see what effect post-translational protein modifications (like glycation) have on protein corona formation. Data related to the optimization of each analytical technique will be presented.

Characterization of the binding behaviors of biological adsorbates to silver nanoparticles

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.