Simple sugars are an integral component of biological matrices and are routinely characterized by liquid and gas chromatography techniques. Non-structural sugars such as glucose, fructose and sucrose play important roles in plant health, growth and regulation. Direct separation of polar molecules such as sugars are challenging using conventional liquid chromatography techniques. Hydrophilic interaction liquid chromatography (HILIC) which was introduced more than a decade ago showed considerable promise over other techniques by retaining polar molecules in the HILIC phase. Similarly, charge aerosol detection also became popular due to its relatively low cost. Over past few years we have evaluated a few HILIC columns under different mobile phase conditions and compared their detection using corona charge aerosol detection (corona CAD). In the course of our investigations, we found that buffer additives in the mobile phase should be cautiously chosen for HILIC separation. These additives not only alter interactions between the aqueous layer and the analytes impacting separation efficiency but can also suppress the signals in the CAD. In some cases the effects are combined and amplified. After an extensive evaluation, we employed a HILIC-Z (HILIC-Zwitterionic) stationary phase successfully to separate non-structural sugars from standards and a few biological matrices. Detailed strategies and results will be discussed.

Few tenets for characterizing polar biomolecules and amino acids using hydrophilic interaction liquid chromatography with corona charge aerosol detection

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.