Catechins are phenolic compounds found in dietary products such as green tea, red wine, and cocoa. These compounds act as antioxidants and are also involved in the regulation of cellular processes including cell death, extracellular matrix degradation, and multidrug resistance. Epigallocatechin-3-gallate (EGCG) is one of the most common catechins. Previous research has found that EGCG suppresses the progression of breast cancer by decreasing cell proliferation and inhibiting angiogenesis. Levels of microRNAs, small non-coding RNAs involved in regulating gene expression, have been shown to vary after EGCG treatment in breast cancer. Studies have shown that microRNA-182 has been shown to have higher expression levels in triple-negative breast cancer (TNBC) cells than in normal cells. Also, research has shown that miR-182 promotes proliferation and metastasis in TNBC. In this study, we investigated the effect of EGCG in miR-182 expression and levels of its target BRCA1 in TBNC MDA-MB-468 cells. We treated the cells with 25μM and50μM of EGCG for 48 hours. We extracted total RNA and use Taqman assays to determine miR-182 and BRCA1 levels. We found that miRNA-182 levels decreased after EGCG treatment but not significantly (miR-18225μM= 83.4± 39.7% (p=0.381) and miR-18250μM= 70.0± 3.9% (p=0.302) vs. untreated). Interestingly, BRCA1 gene expression increased significantly with EGCG concentrations (BRCA125μM= 166.77±74.50% (p=0.009); BRCA150μM= 150.7± 38.9% (p=0.006) vs. untreated). Our results suggest that BRCA1 expression can be modulated by treatment with EGCG, and that is possible that miR-182 has a partial role in regulating the expression of this gene as a response to treatment.<br/>

BRCA1 and miR-182 expression after epigallocatechin-3-gallate exposure in triple negative breast cancer cells

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.