Per- or polyfluoroalkyl compounds (PFASs) are a broad class of synthetic chemicals used in a variety of industrial and consumer applications such as in fire extinguishing agents and hydrophobic surfactants. The multiple carbon-fluorine bonds contribute to these compounds being chemically and thermally stable. Many treatment processes are being explored to remove PFAS in the aquatic systems. To assess efficiency of treatment processes, biological tests are needed to assess the changes in the toxicity of the PFAS-contaminated water after treatment. Accumulation of PFASs in humans has been linked to decreased levels of testosterone and increased levels of estrogens. Therefore, this study will determine the feasibility of using an adrenocarcinoma cell line, H295R, as a bioassay to reflect the endocrine disrupting effects of PFAS mixtures because H295R can produce 17 endogenous hormones including estrogens and androgens. The mechanism of PFASs toxicity is largely unknown with respect to which forms of PFASs may cause adverse effects, and whether the toxicity of these compounds is additive, synergistic, or antagonistic. The effect of the structural features of the different forms of PFASs, such as branching in the chemical structure, chain-length of the carbon backbone, or the type of head groups on the elicited response is poorly understood. The specific aim of this study is to determine the relative endocrine disrupting effects of model PFASs, perfluorooctanoic acid (PFOA) and perfluorooctane sulfonic acid (PFOS) in a model cell line, H295R. In this study, H295R were grown in a hormone free supplemented media. H295R cells were then exposed to nontoxic concentrations (1uM and 10uM) of PFOS and PFOA for 24 hours before the cellular media was collected. Solid phase extraction (SPE) was performed using an Oasis HLB cartridge to reduce matrix effects and to concentrate the hormones in the samples. Quantitative analysis of these hormones was performed using an optimized liquid chromatography tandem mass spectrometry method. This approach can successfully detect hormone concentrations in the cellular media to at least 1 ng/L , which can be used to determine changes in hormone concentrations in H295R cells caused by exposure to PFAS. This bioassay can potentially be useful for assessing changes in the endocrine disrupting effects of water that contain mixtures of PFAS without <i>a priori</i> knowledge of the chemical composition

Assessment of endocrine disrupting effects of per- or polyfluoroalkyl substances on the H295R adrenocarcinoma cell line

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.