Chinese hamster ovary (CHO) cells are the industrial workhorses for recombinant protein production. Despite their importance, very little is known about the molecular and gene regulatory mechanisms that control cellular phenotypes during the bio-manufacturing process. While the cells grow and produce the protein in the bioreactor, it is expected that the expression pattern of genes will change and that such changes may give indications of the cellular state in terms of viability, growth, and productivity. Further, perturbations in the process can also lead to changes in expression patterns. In one of our recent studies, we analyzed the dynamics of gene expression changes during a fed-batch process. The changes in gene expression profiles were examined across key process steps such as a temperature-shift and feed additions. Further, we examined the changes in gene expression patterns in response to variations in the levels of a trace metal. Pooling the transcriptome data, we generated clusters of genes having similar gene expression patterns generating an expression baseline for future comparisons and provide a more nuanced understanding of the changes in cellular physiology during a fed-batch process. Building such datasets will help us link various phenotypic traits to relevant biomarkers and better equip us to respond effectively to future manufacturing challenges.

Studying the dynamics of gene expression patterns in fed-batch processes to gain a better understanding of process

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.