We have previously determined the presence of five catechin derivatives and caffeine in Chinese green tea extracts by high performance liquid chromatography coupled with photodiode array detection and electrospray ionizationmass spectrometry (HPLC-PDA-MS). In this project, we will expand to quantitative analysis of these analytes in extracts of green tea purchased in local supermarkets. Catechins are plant-based flavonoids credited with an antioxidant activity and caffeine is a natural stimulant. This project aims to determine the correlation of the amount of these analytes with the price and the manufacturer of a green tea. The separation was carried out on a silica-C18 Brownlee Choice column with an aqueous methanol gradient. The mobile phase was enriched with 0.1% acetic acid for an enhanced positive mode electrospray ionization response. The identity of analytes was confirmed by comparing retention times, UV, MS and MS/MS spectra with corresponding standards. The quantity of catechin, (–)-epigallocatechin, (–)-epigallocatechin 3-gallate, and caffeine was determined using calibration curves of corresponding standards.

Qualitative and quantitative analysis of caffeine and selected catechin derivatives in various green tea extracts by HPLC-PDA-MS

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.