Resistive-pulse sensing with solid-state nanopore and nanopipette devices holds significant promise for a range of applications in biosensing, sequencing and other analytical applications.[1,2] Building on systematic improvements on different levels, including a better understanding of the translocation process [3] and the development of faster detection electronics,[4-7] the idea of using long DNA 'carriers' as analytical platform also facilitates measurements in mixtures and allows for a degree of multiplexing at the molecular level.[8-10] Accordingly, DNA carriers can then be modified to contain capture probes for proteins, DNA and other analytical targets. As an example, we built a DNA architecture based on molecular self-assembly, consisting of a 7.2 kbp dsDNA carrier with ~100 nt single-stranded (ss) DNA capture probes specific to a target associated with antibiotic resistance in Tuberculosis.[10] Subsequent translocation experiments in nanopipettes allowed for the detection of their hybridisation state, as a DNA assay 'on a string' with significant multiplexing capabilities. In addition to presenting these specific results, I will also discuss the general implications of sensor operation in a broader analytical context.

Resistive pulse sensing and a single-molecule DNA assay on a string

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.