Citral (C<sub>10</sub>H<sub>16</sub>O) is a chemical compound found primarily in the essential oils of lemongrass. The purpose of this investigation is to determine if citral is activating apoptosis by examining the cleavage of PARP in cells treated with the compound. Preliminary research has shown that citral is toxic to M624 human melanoma cells. In this study, M624 cells were treated with citral in concentrations of 0.25mM, 0.375mM, and 0.5mM and incubated for 3 hours. Cells were lysed and samples were analyzed through SDS-PAGE gel electrophoresis and western blotting for the cleavage of PARP, which is a signal that apoptosis has been activated.

Effects of citral on PARP cleavage in M624 human melanoma cells

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.