The selectivity of base pairing and programmable sequencing of DNA as a construction material allows for the controlled nanoscale folding of two- and three-dimensional shapes, with enormous potential in applications such as drug-delivery, molecular computing and bio-sensing. However, current techniques to purify DNA origami from precursors have shown low yields, low scalability, and generally lack optimization for automation. Herein, the development of capillary electrophoresis (CE) as a purification technique for DNA origami is described and shown to be a promising alternative to current methods. DNA nanostructures of various morphology were purified from excess staple and scaffold precursors used in synthesis. Capillary zone electrophoresis and capillary transient isotachophoresis were two modes of CE employed, where origami were labelled with non-covalent intercalating dyes from the SYBR family. Buffer composition, pH, as well as sample injection volume, and separation voltage were manipulated to obtain a high quality and reproducible separation of formed structures from precursors. Three non-covalent SYBR dyes were also characterized by fluorescence spectroscopy to determine binding effects. Fraction collection procedures were optimized for reproducibility and yield, where purified origami samples were validated by offline AFM imaging.

Purification of DNA origami with capillary electrophoresis

Macromolecular Chemistry: The Second Century

[Author Benefits](https://scimeetings.acs.org/?utm_source=pubs_content_marketing&utm_medium=website&utm_campaign=0320_MCF_NPI_Launch_Spring_Homepage&ref=pubs_content_marketing)

[How to use SciMeetings](https://storage.googleapis.com/dl.morressier.com/acs/SciMeetings-ACSSpring2020-HowToGuide_M3020-Final.pdf)

[Frequently Asked Questions](https://storage.googleapis.com/dl.morressier.com/acs/SciMtng_ACSNMSp2020_FAQ_M3120_Final.pdf)

ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.