We annotated and analyzed the genome of Mycobacterium phage TDanisky. TDanisky was discovered in 2015 at James Madison University in Virginia. The phage is a member of cluster F and subcluster F1. TDanisky has morphology of <i>Siphoviridea</i> with a long and non-contractile tail. It has a genome length of 56,275 bps with 112 predicted genes. We used DNA master, BLAST, HHpred, GeneMark, Phamerator, and other programs to analyze the genes and identify homologies. The closest relatives of TDanisky are Mycobacterium phages Sparkdehily, Saal, and SwaggPiglett. We observed that TDanisky has mainly forward genes with exception of genes 10, 26, 39-45 and 47-49 which are reversed. In addition, we further compare TDanisky with other members of F1 subcluster ArcusAngelus, Brocalys, Cabrinians, Galatic, Kersh, Kimberlium, Plumber and ShiLan, the gene content similarity varies from 39.7% to 78.91%. Here we were report the functional annotations of TDanisky and additional analysis of specific genes. We were interested in the genes with homologies to WhiB, which is a transciptional regulator. Phages from the F1 subclaster have from one to five WhiB genes. WhiB of TDanisky shares over 40% homology with the WhiB of Mycobacterium smegmatis its host.

Analysis of  WhiB genes in the genome of mycobacterium phage TDanisky

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.