Since arsenic (As) is extremely toxic metal, its contamination of drinking water sources was estimated to affect human health over 144 million people around the world. The traditional methods for As detection require sophisticated, expensive and bulky equipment, specialize expertise for operation, have a high operating cost. They are not suitable for on-site analysis. Hence, there is a need for a simple, low-cost, and on-site technology to determine As. As has two common oxidation states: arsenate (As(V)) and arsenite (As(III)). Most of the simple analytical methods and sensors have been developed to detect As(III) or total As. While it is more challenging to detect As(V), there are a few studies to detect As(V). However, in these studies, As(V) concentration in environmental water has not been determined because of the lack of selectivity. In this study, we developed a simple and selective analytical method for As(V) using a carboxyfluorescein (FAM)-labeled DNA and cerium oxide nanoparticles (CeO2NPs) and determined As(V) concentrations in groundwater. CeO2NPs has been used to adsorb DNA and quench FAM. As(V) could be adsorbed onto NPs, desorbed DNA and recover FAM. The optimal concentrations of CeO2NPs and FAM-labeled DNA and incubation time were determined to be 15 μg/mL, 40 nM and 6 min, respectively. Under the optimal conditions, a calibration curve of our method was created. The LOD was calculated to be 25 μg-As/L. According to the selectivity test, our method had a high selectivity toward As(V). Using the calibration curve, we determined As(V) concentrations in groundwaters (Fig.1). The relative errors in the As(V) concentrations determined by our method and ICP-MS were within 30%. Therefore, we concluded that our method might be applicable to As(V) monitoring in groundwater with a variety of ions at high concentration. 

Development of a simple analytical method for determination of Arsenate using FAM-labeled DNA and cerium oxide nanoparticles

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.