Lovastatin is the first commercialized drugs for hypercholesterolemia and still serving as a precursor for the next generation drugs, such as, wuxistatin and simvastatin. Lovastatin was produced as a secondary metabolite by Monascus purpureus. The present work is focused on studying the effect of culture conditions on the production of lovastatin by Monascus purpureus and predominant flux pattern of the organism to propose the robust biosynthesis pathway of lovastatin. Metabolic footprinting techniques is a quantitative analysis of exometabolites that was used to profile the extracellular metabolites of an organism. The same technique has been used to find the predominant metabolic pathway of the organism and the effect of culture conditions on the flux pattern of the organism under static and shake culture conditions. The flux pattern of the organism is different in those culture condition. By products, glycerol and ethanol was also produced along with TCA intermediates. The assimilation pattern of the secondary metabolites in the shake culture condition is different from that in static culture condition. It was found that the culture condition and the morphology of the organism has also played an important role in the different level of expression of lovastatin in static and shake cultures. It could also reason the possible choice of medium component of the fermentation. Further, the observation was developed into dynamic metabolic models to validate the observation. 

Medium as a message for metabolomic analysis. Metabolic footprinting: Tool to understand the effect of culture condition on production of lovastatin by <i>Monascus purpureus</i>

Macromolecular Chemistry: The Second Century

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ACS Spring 2020 National Meeting & Expo

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Medium as a message for metabolomic analysis. Metabolic footprinting: Tool to understand the effect of culture condition on production of lovastatin by Monascus purpureus