Synergistic interaction between polyphenolic compounds (PC) and probiotics may improve the proliferation of probiotics and the absorption of PC and their metabolites in the gut. In this context, encapsulation of jabuticaba peel extract (JPE) using cells-based carriers, such as <i>Lactobacillus</i> cells, was evaluated as a strategy to simultaneously deliver PC and probiotics. Jabuticaba peel was selected as it has high levels of PC and it is a by-products of juice processing industry. This research focused on the recovery of total polyphenolic compounds (TPC) from jabuticaba peel and their infusion in live probiotics cells using passive and vacuum infusion methods. The recovery of TPC was performed using solutions with different concentration of ethanol (0%, 25% and 50% v/v) at 40 °C for 2 h. The measured TPC in JPE was between 36 to 52 mg GAE/g. Vacuum infusion using JPE in 25% of ethanol solution provided the maximum loading of PC (8 mg GAE/g of cells) in probiotic cells in less than 5 minutes without significantly influencing cell viability. Vacuum infusion using JPE dissolved in water provided the loading of PC below 2 mg GAE/g of cells. The presence of ethanol in the encapsulating mixture improved loading by increasing the solubility of PC as well as their partitioning in cells during vacuum infusion. The loading of PC using passive infusion ranged between 1.5 to 4.5 mg GAE/g of cells after 24 hours of incubation. This value was 2 – 4-fold lower than that obtained via vacuum infusion. Probiotic cells after infusion of JPE were viable, counts ranged from 7.8 to 11 log CFU/g. Release of encapsulated compounds from bacterial cells were measured in simulated gastrointestinal fluids. <i>Lactobacillus</i> cells released from 20% to 40% of PC. Overall, vacuum infusion is a promising technique for the encapsulation of plant extract in probiotic cells and their delivery to the gut. This research provides a novel platform to evaluate the synergistic interactions between probiotic and PC.

Infusion of jabuticaba peel extract in probiotic cells by passive and vacuum mechanisms

ACS Fall 2020 Virtual Meeting

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.