FlowVPE (C Technologies) is a variable path length UV-VIS spectrophotometer that can be used to accurately measure protein concentrations across a much larger range than typical inline UV detectors on FPLC instruments. The sensitivity of the instrument is sufficient to monitor the capture and breakthrough of product using clarified harvest as the feed stream on Protein A columns. KBI Biopharma has generated data for multiple molecules demonstrating the comparability of FlowVPE UV breakthrough data to offline ProA-HPLC titer analysis for determination of Protein A resin dynamic binding capacity. Application of FlowVPE enables real-time data generation, reducing analytical support requirements for sizing ProA capture columns. This capability results in both time and cost savings in an accelerated downstream purification process development workflow.

Exploring efficiency gains with the implementation of FlowVPE into accelerated downstream process development

ACS Fall 2020 Virtual Meeting

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.