The toxicology in the 21st century (Tox21) program, a collaboration of the National Institutes of Health, Environmental Protection Agency, and Food and Drug Administration, has utilized a quantitative high throughput screening (qHTS) approach to profile thousands of environmental chemicals against a battery of in vitro assays. Millions of data points generated from Tox21 screening were made publicly accessible. These rich datasets provide researchers with opportunities for further data mining, understanding compound action, and prioritizing compound for further in-depth studies. In order to generate high quality and reproducible data, the assay selection, optimization, validation, and screening performance play a critical role in toxicological testing. This presentation will highlight these critical components in assay development and screening process. Several examples of in vitro assays that are available for compound toxicity assessment will be evaluated in detail. The screening results from these in vitro assays will be useful for prioritizing chemicals for further in-depth mechanism-based toxicity testing.

Profiling environmental chemicals using in vitro qHTS methods

ACS Fall 2020 Virtual Meeting

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