The Annonaceous acetogenins are a class of natural products isolated from the plant family Annonacea and exhibit a broad range of biological activity, including anticancer, antimicrobial, pesticidal and neurotoxic. Within the class of Annonaceous acetogenins is annonacin, a lipophilic mitochondrial complex I inhibitor which is more toxic than rotenone in mammalian neurons and promotes microtubule-associated protein tau aggregation and nigrostriatal neuronal death in vitro and in vivo. The Parkinsons-like neurotoxicity associated with consumption of the fruit of Annona muricata (the soursop), has been traced to the occurrence of annonacin in the fruit extracts. Using mass spectroscopy and NMR methods, we found that annonacin is present in the fruit pulp of the North American paw paw Asimina triloba in sufficient quantities to support  ongoing neurological studies and have developed a protocol for extracting and isolating the material in reasonable purity. The materials, methods and protocols for extraction of the natural material from Asimina triloba will be presented.

Isolation of annonacin from the fruit pulp of <i>Asimina triloba</i>

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239th National Meeting (2010)

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Isolation of annonacin from the fruit pulp of Asimina triloba