F�rster resonant energy transfer (FRET) is a spectroscopic tool capable of determining the molecular distances and the presence of molecular complexes.  By using a pair of appropriate fluorophores, the FRET phenomenon can be used as an analytical tool to probe molecular interactions between molecules usually between 1-10 nm.  In our initial study, a water soluble cellulose derivative � carboxymethyl cellulose (CMC) was employed with cellulase as a model system for subsequent liquid/solid systems.  FRET technique was for the first time applied to study the cellulase-cellulose interaction upon efficient labeling of CMC in organic solvent.  After the mixing of labeled- cellulase and CMC conjugates, FRET phenomena were successfully observed in homogeneous aqueous solution via steady-state fluorescence spectroscopic method.  The temperature dependence of cellulase binding to CMC was revealed by the experiment results.  This developed method could be used for further investigation of cellulase-cellulose interaction in liquid phase.

Study cellulase-cellulose interaction using FRET

239th National Meeting (2010)

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